OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Rapid assessment of metabolic activity in marine microalgae : application in ecotoxicological tests and evaluation of water quality

Gilbert, Franck and Galgani, François and Cadiou, Yvon Rapid assessment of metabolic activity in marine microalgae : application in ecotoxicological tests and evaluation of water quality. (1992) Marine Biology, vol. 112 (n° 2). pp. 199-205. ISSN 0025-3162

[img] (Document in English)

PDF (Publisher's version) - Depositor and staff only - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
711kB

Official URL: http://dx.doi.org/10.1007/BF00702462

Abstract

A new method for the assessment of the effects of several contaminants on marine microalgae,Tetraselmis suecica (Kyling) Butcher,Skeletonema costatum (Grev.) Cleve, andProrocentrum lima (Ehrenberg) Dodge was developed in 1990. The method is based on the measurement of cell esterase activity using a fluorimetric stain, fluorescein diacetate (FDA), selected from amongst three stains (FDA, Neutral Red, thiazolyl tetrazolium bromure) for its higher sensitivity. Biochemical (K m,V max) and physiological (growth, specific activity) aspects of the enzymatic activity as revealed by the FDA method are discussed. Different categories of compounds (weed-killers, insecticides, metals) and some water samples from Seine Bay were tested for their toxic effects on microalgae. Experiments were performed on microplates using a fluorimetric microplate reader. The various steps of the experiments and data-processing were controlled by software. Applications of the system to rapid ecotoxicological tests (determination of the IC50, i.e., the concentration at which 50% inhibition of growth occurs) and to the assessment of environment quality by studying the toxic effect of water samples on microalgae are proposed.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at http://link.springer.com The original PDF of the article can be found at Marine Biology website : http://link.springer.com/article/10.1007%2FBF00702462
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut Français de Recherche pour l'Exploitation de la MER - IFREMER
Laboratory name:
Statistics:download
Deposited By: Franck Gilbert

Repository Staff Only: item control page