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Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader

Galgani, François and Cadiou, Yvon and Gilbert, Franck Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader. (1992) Ecotoxicology and Environmental Safety, vol. 23 (n° 2). pp. 237-243. ISSN 0147-6513

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Official URL: http://dx.doi.org/10.1016/0147-6513(92)90061-7

Abstract

A system is described for determination of LC50 or IC50 by an iterative process based on data obtained from a plate reader using a marine unicellular alga as a target species. The esterase activity of Tetraselmis suesica on fluorescein diacetate as a substrate was measured using a fluorescence titerplate. Simultaneous analysis of results was performed using an iterative process adopting the sigmoid function View the MathML source (dose of toxicant/IC50)slope for dose-response relationships. IC50 (± SEM) was estimated (P < 0.05). An application with phosalone as a toxicant is presented.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut Français de Recherche pour l'Exploitation de la MER - IFREMER
Laboratory name:
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Deposited By: Franck Gilbert
Deposited On:28 Sep 2012 11:35

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