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New dithiolopyrrolone antibiotics induced by adding sorbic acid to the culture medium of Saccharothrix algeriensis NRRL B-24137

Merrouche, Rabiaa and Bouras, Noureddine and Coppel, Yannick and Mathieu, Florence and Sabaou, Nasserdine and Lebrihi, Ahmed New dithiolopyrrolone antibiotics induced by adding sorbic acid to the culture medium of Saccharothrix algeriensis NRRL B-24137. (2011) FEMS Microbiology Letters, vol. 318 (n° 1). pp. 41-46. ISSN 0378-1097

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Official URL: http://dx.doi.org/10.1111/J.1574-6968.2011.02246.X

Abstract

Dithiolopyrrolone antibiotics, produced by several microorganisms, are known for their strong antimicrobial activities. This class of antibiotics generated new interest after the discovery of their anticancer and antitumor properties. In this study, four new antibiotics were purified from the fermentation broth of Saccharothrix algeriensis NRRL B-24137 and characterized as dithiolopyrrolone derivatives. These new dithiolopyrrolone antibiotics were induced by adding sorbic acid, as precursor, at a concentration of 5 mM to the semi-synthetic medium. The analysis of the induced antibiotics was carried out by HPLC. The maximal production of the antibiotics PR2, PR8, PR9 and PR10 was 0.08±0.04, 0.21±0.04, 0.13±0.03 and 0.09±0.00 mg L−1, respectively, obtained after 8 days of fermentation. The chemical structures of these antibiotics were determined by 1H- and 13C-nuclear magnetic resonance, mass and UV-visible data. The four new dithiolopyrrolone antibiotics – PR2, PR8, PR9 and PR10 – were characterized, respectively, as crotonyl-pyrrothine, sorbyl-pyrrothine, 2-hexonyl-pyrrothine and 2-methyl-3-pentenyl-pyrrothine. The minimum inhibitory concentrations of the new induced antibiotics were determined.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Laboratory name:
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Deposited By:Audrey LEFEVRE

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