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Identification and partial characterization of antifungal and antibacterial activities of two Bacillus sp. strains isolated from salt soil in Tunisia

Sihem, Ben Maachia and Rafik, Errakhi and Mathieu, Florence and Mohamed, Chérif and Lebrihi, Ahmed Identification and partial characterization of antifungal and antibacterial activities of two Bacillus sp. strains isolated from salt soil in Tunisia. (2011) African Journal of Microbiology Research, vol. 5 (n° 13). pp. 1599-1608. ISSN 1996-0808

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Official URL: http://dx.doi.org/10.5897/AJMR11.073

Abstract

Two Bacillus sp. strains (B29 and B27) isolated from soil in the South of Tunisia were tested for their abilities to produce antimicrobial compounds. Both strains showed antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi. The produced compounds were extracted by using four different solvents. The hexane solvent allowed to obtain maximum of activity of the strain B29. The activity of the strain B27 was not elucidated by the four solvents used. Bio-autography results of B29 hexane extract revealed presence of different antibiotics and antifungal compounds with different Rf values of 0.3 and 0.76 for antifungal compounds and of 0.12, 0.14, 0.19 and 0.3 for antibacterial ones. Two active fractions were isolated from the culture broth of the strain B29 by semi-preparative high performance liquid chromatography (HPLC). The partial sequencing of the 16S rDNA gene was used to identify the two Bacillus strains. They may be assigned to new Bacillus species

Item Type:Article
Additional Information:Thanks to Academic Journals editor. The definitive version is available at http://www.academicjournals.org The original PDF of the article can be found at : http://www.academicjournals.org/AJMR/abstracts/abstracts/abstract%202011/4July/Sihem%20et%20al.htm
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Other partners > Institut National Agronomique de Tunisie - INAT (TUNISIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > Institution de la Recherche et de l'Enseignement Supérieur Agricoles - IRESA (TUNISIA)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Agronutrition (FRANCE)
Laboratory name:
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Deposited By: Audrey LEFEVRE

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