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Electrochemical impedance spectroscopy to study physiological changes affecting the red blood cell after invasion by malaria parasites

Ribaut, Clothilde and Reybier, Karine and Reynes, Olivier and Launay, Jérôme and Valentin, Alexis and Fabre, Paul-Louis and Nepveu, Françoise Electrochemical impedance spectroscopy to study physiological changes affecting the red blood cell after invasion by malaria parasites. (2009) Biosensors and Bioelectronics, vol. 24 (n° 8). pp. 2721-2725. ISSN 0956-5663

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Official URL: http://dx.doi.org/10.1016/J.BIOS.2008.12.018

Abstract

The malaria parasite, Plasmodium falciparum, invades human erythrocytes and induces dramatic changes in the host cell. The idea of this work was to use RBC modified electrode to perform electrochemical impedance spectroscopy (EIS) with the aim of monitoring physiological changes affecting the erythrocyte after invasion by the malaria parasite. Impedance cell-based devices are potentially useful to give insight into cellular behavior and to detect morphological changes. The modelling of impedance plots (Nyquist diagram) in equivalent circuit taking into account the presence of the cellular layer, allowed us pointing out specific events associated with the development of the parasite such as (i) strong changes in the host cell cytoplasm illustrated by changes in the film capacity, (ii) perturbation of the ionic composition of the host cell illustrated by changes in the film resistance, (iii) releasing of reducer (lactic acid or heme) and an enhanced oxygen consumption characterized by changes in the charge transfer resistance and in the Warburg coefficient characteristic of the redox species diffusion. These results show that the RBC-based device may help to analyze strategic events in the malaria parasite development constituting a new tool in antimalarial research.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at : http://www.sciencedirect.com/science/article/pii/S0956566308006477
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA
Université de Toulouse > Institut Supérieur de l'Aéronautique et de l'Espace - ISAE
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Université de Toulouse > Université de Toulouse II-Le Mirail - UTM
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Deposited By: Audrey LEFEVRE

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