OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars

Arshad, Muhammad and Silvestre, Jérôme and Merlina, Georges and Dumat, Camille and Pinelli, Eric and Kallerhoff, Jean Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars. (2012) Plant Cell, Tissue and Organ Culture, vol. 108 (n°2). pp. 315-322. ISSN 0167-6857

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
550kB

Official URL: http://dx.doi.org/10.1007/s11240-011-0045-1

Abstract

Shoot organogenesis from mature leaf tissues of two scented Pelargonium capitatum cultivars, ‘Attar of Roses’ and ‘Atomic Snowflake’, grown in the greenhouse, were optimized in the presence of thidiazuron (TDZ). The protocol involved preculture of leaf sections on basal Murashige and Skoog (MS) medium supplemented with 10 lM TDZ, 4.4 lM of 6-benzyladenine (BA) and 5.4 lM a-naphtaleneacetic acid (NAA) for a period of 2 weeks and followed by subculture of explants to a fresh medium containing 4.4 lM BA and 5.4 lM NAA. Frequency of regeneration reached approximately 93% for both cultivars, with the induction of more than 100 shoots per explant. Regenerated plantlets were rooted on half-strength MS medium supplemented with 4.4 mM sucrose and 8.6 lM of Indole-3-acetic acid (IAA). All regenerated shoots from both cultivars developed roots when transferred to organic soil mix, acclimatized, and successfully transferred to greenhouse conditions. When regenerated shoots were transferred to hydroponic conditions, frequency of survival was 76.2 and 61.9% for ‘Attar of Roses’ and ‘Atomic Snowflake’, respectively.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at http://www.springerlink.com/content/pl3t422186375210/?MUD=MP
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Laboratory name:
Statistics:download
Deposited By: Nadia Kelmouss
Deposited On:11 Jul 2012 15:43

Repository Staff Only: item control page