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Comparative Evaluation of Genotoxicity of Captan in Amphibian Larvae (Xenopus laevis and Pleurodeles waltl) Using the Comet Assay and the Micronucleus Test

Mouchet, Florence and Gauthier, Laury and Mailhes, Corinne and Ferrier, Vincent and Devaux, Alain Comparative Evaluation of Genotoxicity of Captan in Amphibian Larvae (Xenopus laevis and Pleurodeles waltl) Using the Comet Assay and the Micronucleus Test. (2006) Environmental Toxicology, vol. 21 (n° 3). pp. 264-277. ISSN 1520-4081

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Official URL: http://dx.doi.org/10.1002/tox.20180

Abstract

Captan (N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide) is a fungicide used to inhibit the growth of many types of fungi on plants used as foodstuffs. The toxic and genotoxic potentials of captan were evaluated with the micronucleus test (MNT; AFNOR,2000) and the comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that captan was toxic (1) to Xenopus larvae exposed to from 2 mg/L to 125 or 62.5 μg/L, depending on the nature of the water [reconstituted water containing mineral salts or mineral water (MW; Volvic®, Danone, France)] and (2) to Pleurodeles exposed to from 2 mg/L to 125 μg/L in both types of water. The MNT results obtained in MW showed that captan (62.5 μg/L) was genotoxic to Xenopus but not genotoxic to Pleurodeles at all concentrations tested. CA established that the genotoxicity of captan to Xenopus and Pleurodeles larvae depended on the concentration, the exposure times, and the comet parameters (tail DNA, TEM, OTM, and TL). The CA and MNT results were compared for their ability to detect DNA damage at the concentrations of captan and the exposure times applied. CA showed captan to be genotoxic from the first day of exposure. In amphibians, CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by captan. © 2006 Wiley Periodicals, Inc. Environ Toxicol 21: 264–277, 2006

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Other partners > Ecole Nationale des Travaux Publics de l'Etat - ENTPE (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Centre universitaire de formation et de recherche Jean-François Champollion - JFC (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Université de Toulouse > Université de Toulouse II-Le Mirail - UTM
Université de Toulouse > Université de Toulouse I-Sciences Sociales - UT1
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Deposited By: Nadia Kelmouss
Deposited On:01 Jun 2012 12:39

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