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Biomonitoring of the genotoxic potential (micronucleus assay) and detoxifying activity (EROD induction) in the River Dadou (France), using the amphibian Xenopus laevis

Gauthier, Laury and Tardy, Emmanuelle and Mouchet, Florence and Marty, Josette Biomonitoring of the genotoxic potential (micronucleus assay) and detoxifying activity (EROD induction) in the River Dadou (France), using the amphibian Xenopus laevis. (2004) Science of the Total Environment, vol. 323 (n° 1-3). pp. 47-61. ISSN 0048-9697

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Official URL: http://dx.doi.org/10.1016/j.scitotenv.2003.10.014

Abstract

Within the framework of a general survey of the water quality of the river Dadou (Tarn, France), different physicochemical parameters were measured and an inventory of the fish population was made along the water course, around the Rassisse dam.With the aim of monitoring the potential genotoxic effects and the detoxifying activities induced in organisms exposed to the river water, two in vivo bioassays were performed in laboratory experiments, using larvae of the amphibian Xenopus laevis.The first was the micronucleus test, using red blood cells, and the second the assay of ethoxyresorufin-O-deethylase (EROD) induction in the liver of exposed animals.Eight water samples were taken from the river and at outlet points from the two major industrial activities of the studied section of the water course: a spar-fluor mine and a water treatment plant.Genotoxic impact and EROD induction were measured in the larvae.The effluent of the filter-washing process from the water treatment plant was found to be particularly genotoxic, even after dilution in pure reconstituted water, but no particular genotoxicity was found, either in Dadou river water, or in the effluents from the mine.On the other hand, most of the water samples tested produced a clear induction of EROD activity compared to the level of enzymatic activity found in the liver of larvae reared in the river water sampled upstream of the industrial activities.These results were interpreted taking into account (i) the high concentrations of pollutants (fluorine and manganese) measured in the river water, (ii) the very low population levels inventoried in the downstream section of the river and (iii) the possible interactions between the substances present in the river water, particularly the classical EROD inducers PAHs and PCBs.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com/science/article/pii/S0048969703005989
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Other partners > Centre universitaire de formation et de recherche Jean-François Champollion - JFC (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
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Deposited By:Nadia Kelmouss

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