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Evaluation of the genotoxic and teratogenic potential of a municipal sludge and sludge-amended soil using the amphibian Xenopus laevis and the tobacco: Nicotiana tabacum L. var. xanthi Dulieu

Chenon, Pascale and Gauthier, Laury and Loubières, Pascale and Séverac, Alain and Delpoux, Marcel Evaluation of the genotoxic and teratogenic potential of a municipal sludge and sludge-amended soil using the amphibian Xenopus laevis and the tobacco: Nicotiana tabacum L. var. xanthi Dulieu. (2003) Science of the Total Environment, vol. 301 (n° 1-3). pp. 139-150. ISSN 0048-9697

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Official URL: http://dx.doi.org/10.1016/S0048-9697(02)00287-5

Abstract

The toxic, genotoxic and teratogenicpotential of amunicipal sewage sludge was assessed using the micronucleus assay on the larvae of the amphibianXenopuslaevis and with the tobacco somatic mutation test using the yellow–green xanthiDulieu mutant a1+/a1 a2+/a2. The teratogenicpotential was assessed by means of the Frog Embryo Teratogenesis Assay-Xenopus (FETAX). Various doses of the pasty sludge added to a crop soil were tested using the three bioassays. The test systems were performed either directly with sludge or sludge-amendedsoil samples (plant model) or with aqueous extracts (aquatic animal model). Using the tobacco model, we found no mutagenic impact of the soilamended with the sludge, perhaps because the clay-like nature of the soil, with its high adsorption capacity, may have prevented the contaminants from reaching the target. All leachates of amendedsoils produced a significant size reduction in Xenopus embryos. Depending on the soil/sludge ratio, some leachates were found to be genotoxic but were never teratogenic. This battery of in vivo test systems enabled us to estimate the global long-term effects under agricultural conditions with various genetic endpoints on ecologically relevant organisms characteristic of the aquatic and terrestrial compartments.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM
Other partners > Centre universitaire de formation et de recherche Jean-François Champollion - JFC (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Laboratory name:
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Deposited By: Nadia Kelmouss

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