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Ochratoxin A in Roasted Coffee from French Supermarkets and Transfer in Coffee Beverages: Comparison of Analysis Methods

Tozlovanu, Mariana and Pfohl-Leszkowicz, Annie Ochratoxin A in Roasted Coffee from French Supermarkets and Transfer in Coffee Beverages: Comparison of Analysis Methods. (2010) Toxins, vol. 2 (n° 8). pp. 1928-1942. ISSN 2072-6651

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Official URL: http://dx.doi.org/doi:10.3390/toxins2081928

Abstract

The OTA content of 30 roasted coffees purchased in French supermarkets was evaluated by two validated different methods: one using immunoaffinity column (IAC) clean-up after alkaline extraction; the second using toluene extraction under acidic conditions. OTA recoveries (0.5 to 5 µg/kg) ranged from 16–49% with the alkaline extraction method and 55–60% with the acidic method. OTA recoveries from prepared beverages were similar with all methods (75–80%). All samples containing OTA ranged from trace (<LOQ) to 11.9 µg/kg. About 20 to 140% of OTA passed through the beverages. Recoveries of over 100% of OTA in beverages were due to three types of interferences: (i) formation of open-ring OTA (OP-OA) during alkaline extraction, (ii) isomerization of OTA during roasting, and (iii) presence of the nonchlorinated analogue OTB. The first two types of interference generate OTA derivatives that are not recognized by OTA antibodies, while OTB cross-reacts with OTA-antibodies. These analytical problems will seriously impact the amount of OTA detected, especially at the levels close to the limits from the EU legislation. Underestimation of OTA could be highly dangerous for health.

Item Type:Article
Additional Information:Thanks to MDPI editor. The definitive version is available at http://www.mdpi.com The original PDF of the article can be found at : http://www.mdpi.com/2072-6651/2/8/1928
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
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Deposited By: Audrey LEFEVRE

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