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Chloroquine-enhanced gene delivery mediated by carbon nanotubes

Sanz, Vanessa and Tîlmaciu, Carmen and Soula, Brigitte and Flahaut, Emmanuel and Coley, Helen M. and Silva, S. Ravi P. and McFadden, Johnjoe Chloroquine-enhanced gene delivery mediated by carbon nanotubes. (2011) Carbon, vol. 49 (n° 15). pp. 5348-5358. ISSN 0008-6223

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Official URL: http://dx.doi.org/10.1016/j.carbon.2011.08.001

Abstract

Polyethyleneimine-coated double-walled carbon nanotubes (DWCNTs) were used for dual gene and drug delivery, after loading the DWCNTs with the drug chloroquine, a lysosomotropic compound that is able to promote escape from the lysosomal compartment. Different forms of functionalization of the DWCNTs were examined in order to optimize this system. They included the testing of different treatments on DWCNTs to optimize the loading and delivery of chloroquine and the selection of a cationic polymer for coating the DWCNTs for optimum DNA binding and delivery. An acid oxidation treatment of DWCNTs was selected for optimum chloroquine loading together with polyethyleneimine as optimum cationic coating agent for plasmid DNA binding. Optimization of the conditions for choroquine-enhanced gene delivery were developed using luciferase expression as a model system. We have demonstrated that chloroquine-loading increases the ability of polyethyleneimine-coated DWCNTs to deliver functional nucleic acid to human cells. Cell viability tests have shown no cytotoxicity of the functionalized DWCNTs at the concentrations needed for optimum gene delivery. These results support the potential applications of this methodology in gene therapy.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original version is available at http://www.sciencedirect.com The original PDF of the article can be found at Carbon website: http://www.sciencedirect.com/science/article/pii/S0008622311006270
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > University of Surrey (UNITED KINGDOM)
Laboratory name:
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Deposited By: Denis Faragou
Deposited On:06 Jun 2012 20:45

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