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Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring

Polard, Thierry and Jean, Séverine and Merlina, Georges and Laplanche, Christophe and Pinelli, Eric and Gauthier, Laury Giemsa versus acridine orange staining in the fish micronucleus assay and validation for use in water quality monitoring. (2011) Ecotoxicology and Environmental Safety, vol. 74 (n°1). pp. 144-149. ISSN 0147-6513

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Official URL: http://dx.doi.org/10.1016/j.ecoenv.2010.08.005

Abstract

This study concerns a comparative analysis of the acridineorange and Giemsastaining procedures for the fish erythrocyte micronucleusassay. The goal was to optimize the assay in the context of field watermonitoring. Fish (Carassius carassius) were exposed to a reference genotoxic agent, cyclophosphamide monohydrate 5 mg l−1 for 2, 4, and 6 days before testing. Slides from each individual were scored using the two procedures. The results show that the assay was more sensitive when acridineorange was used. When slides were Giemsa stained, the presence of ambiguous artefacts, leading to false positives and increasing random variance, reduced the contrast between exposed and control samples. AcridineOrangestaining was then applied in the context of waterqualitymonitoring. Fish were exposed for 4 days to water sampled in two hydrological contexts: basal flow and spring flood. The results show that exposure to spring flood water in an agricultural stream can induce mutagenicity.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
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Deposited By: Nadia Kelmouss
Deposited On:21 May 2012 10:13

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