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Lead-induced genotoxicity to Vicia faba L.roots in relation with metal cell uptake and initial speciation

Shahid, Muhammad and Pinelli, Eric and Pourrut, Bertrand and Silvestre, Jérôme and Dumat, Camille Lead-induced genotoxicity to Vicia faba L.roots in relation with metal cell uptake and initial speciation. (2011) Ecotoxicology and Environmental Safety, vol. 74 (n°1). pp. 78-84. ISSN 0147-6513

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Official URL: http://dx.doi.org/10.1016/j.ecoenv.2010.08.037

Abstract

Formation of organometallic complexes in soil solution strongly influence metals phytoavailability. However, only few studies deal with the influence of metalspeciation both on plant uptake and genotoxicity. In the present study, Viciafaba seedlings were exposed for 6 h in controlled hydroponic conditions to 5 μM of lead nitrate alone and chelated to varying degrees by different organic ligands. Ethylenediaminetetraacetic acid and citric acid were, respectively, chosen as models of humic substances and low weight organic acids present in natural soil solutions. Visual Minteq software was used to estimate free lead cations concentration and ultimately to design the experimental layout. For all experimental conditions, both micronucleus test and measure of leaduptake by plants were finally performed. Chelation of Pb by EDTA, a strong chelator, dose-dependently increased the uptake in V. faba roots while its genotoxicity was significantly reduced, suggesting a protective role of EDTA. A weak correlation was observed between total lead concentration absorbed by roots and genotoxicity (r2=0.65). In contrast, a strong relationship (r2=0.93) exists between Pb2+ concentration in exposure media and genotoxicity in the experiment performed with EDTA. Citric acid induced labile organometallic complexes did not demonstrate any significant changes in leadgenotoxicity or uptake. These results demonstrate that metalspeciation knowledge could improve the interpretation of V. fabagenotoxicity test performed to test soil quality.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Université Catholique de Lille (FRANCE)
Laboratory name:
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Deposited By:Nadia Kelmouss

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