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The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids

Grenier, Bertrand and Bracarense, Ana-Paula and Schwartz, Heidi-Elisabeth and Trumel, Catherine and Cossalter, Anne-Marie and Schatzmayr, Gerd and Kolf-Clauw, Martine and Moll, Wulf-Dieter and Oswald, Isabelle P The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids. (2012) Biochemical Pharmacology, vol. 83 (n° 10). pp. 1465-1473. ISSN 0006-2952

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Official URL: http://dx.doi.org/10.1016/j.bcp.2012.02.007

Abstract

Fumonisins are mycotoxins frequently found as natural contaminants in maize, where they are produced by the plant pathogen Fusarium verticillioides. They are toxic to animals and exert their effects through mechanisms involving disruption of sphingolipid metabolism.Fumonisin B1 (FB1) is the predominant fumonisins in this family. FB1 is converted to its hydrolyzed analogs HFB1, by alkaline cooking (nixtamalization) or through enzymatic degradation. The toxicity of HFB1 is poorly documented especially at the intestinal level. The objectives of this study were to compare the toxicity of HFB1 and FB1 and to assess the ability of these toxins to disrupt sphingolipids biosynthesis. HFB1 was obtained by a deesterification of FB1, with a carboxylesterase. Piglets, animals highly sensitive to FB1, were exposed by gavage for 2 weeks to 2.8 µmol FB1 or HFB1/kg body weight/day. FB1 induced hepatotoxicity as indicated by the lesion score, the level of several biochemical analytes and the expression of inflammatory cytokines. Similarly, FB1 impaired the morphology of the different section of the small intestine, reduced villi height and modified intestinal cytokine expression. By contrast, HFB1 did not trigger hepatotoxicity, did not impair intestinal morphology and slightly modified the intestinal immune response. This low toxicity of HFB1 correlates with a weak alteration of the sphinganine/sphingosine ratio in the liver and in the plasma. Taken together, these data demonstrate that HFB1 does not cause intestinal or hepatic toxicity in the sensitive pig model, and slightly disrupts sphingolipids metabolism. This finding suggests that conversion to HFB1 could be a good strategy to reduce FB1 exposure.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article is available at http://www.sciencedirect.com/science/journal/00062952
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Universität für Bodenkultur Wien (AUSTRIA)
Other partners > Universidade Estadual de Londrina (BRASIL)
Laboratory name:
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Deposited By: martine kolf-clauw
Deposited On:03 May 2012 14:11

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