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Identification and genetic characterization of a gibberellin 2-oxidase gene that controls tree stature and reproductive growth in plum

El-Sharkawy, Islam and El Kayal, Walid and Prasath, D. and Fernandez, H. and Bouzayen, Mondher and Svircev, Antonet M. and Jayasankar, Subramanian Identification and genetic characterization of a gibberellin 2-oxidase gene that controls tree stature and reproductive growth in plum. (2012) Journal of Experimental Botany, vol. 63 (n°3). pp. 1225-1239. ISSN 0022-0957

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Official URL: http://dx.doi.org/10.1093/jxb/err345

Abstract

Several dwarf plum genotypes (Prunus salicina L.), due to deficiency of unknown gibberellin (GA) signalling, were identified. A cDNA encoding GA 2-oxidase (PslGA2ox), the major gibberellin catabolic enzyme in plants, was cloned and used to screen the GA-deficient hybrids. This resulted in the identification of a dwarf plum hybrid, designated as DGO24, that exhibits a markedly elevated PslGA2ox signal. Grafting ‘Early Golden’ (EG), a commercial plum cultivar, on DGO24 (EG/D) enhanced PslGA2ox accumulation in the scion part and generated trees of compact stature. Assessment of active GAs in such trees revealed that DGO24 and EG/D accumulated relatively much lower quantities of main bioactive GAs (GA1 and GA4) than control trees (EG/M). Moreover, the physiological function of PslGA2ox was studied by determining the molecular and developmental consequences due to ectopic expression in Arabidopsis. Among several lines, two groups of homozygous transgenics that exhibited contrasting phenotypes were identified. Group-1 displayed a dwarf growth pattern typical of mutants with a GA deficiency including smaller leaves, shorter stems, and delay in the development of reproductive events. In contrast, Group-2 exhibited a ‘GA overdose’ phenotype as all the plants showed elongated growth, a typical response to GA application, even under limited GA conditions, potentially due to co-suppression of closely related Arabidopsis homologous. The studies reveal the possibility of utilizing PslGA2ox as a marker for developing size-controlling rootstocks in Prunus.

Item Type:Article
Additional Information:Thanks to Oxford Journals editor. The definitive version is available at http://jxb.oxfordjournals.org/content/63/3/1225
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Southern Crop Protection and Food Research Centre (CANADA)
Other partners > Universidad de Oviedo (SPAIN)
Other partners > University of Alberta (CANADA)
Other partners > University of Guelph (CANADA)
Laboratory name:
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Deposited By:Brigitte LAFFORGUE

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