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Development and validation of methods for the trace determination of phthalates in sludge and vegetables

Sablayrolles, Caroline and Montréjaud-Vignoles, Mireille and Benanou, David and Patria, Lucie and Treilhou, Michel Development and validation of methods for the trace determination of phthalates in sludge and vegetables. (2005) Journal of Chromatography A, vol. 1072 (n° 2). pp. 233-242. ISSN 0021-9673

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Official URL: http://dx.doi.org/10.1016/j.chroma.2005.02.074

Abstract

A routine method which is simple, quick and precise has been set up and validated for phthalate analysis in environmental samples (tomato plants and sewage sludges). Six phthalates have been studied simultaneously: dimethylphthalate, diethylphthalate, dibutylphthalate, butylbenzylphthalate, di(ethyl)hexylphthalate and dioctylphthalate. Optimization of sample, solvent extraction uses a Soxtec apparatus and extract purification with an SPE cartridge allows between 90 to 110 % recovery of phthalates. Precise, sensitive and selective identification and quantifying of analytes is by GC-MS in SIM mode. This protocol allows analytes with concentrations as low as 10µg/kg Dry Matter (DM) to be determined from small (1 to 2 g DM) samples. This analytical method has been applied to the phthalate transfer study for agricultural recycling of sludges, where phthalate bioavailability has been studied in aquiculture using two types of experiments. Tomatoes have been grown in containers where the trace organics have been directly introduced as pure substances, and in a second experiment under the same growth conditions, sewage sludge has replaced the pure substances. Transfer of these trace organics has been followed into the various parts of the tomato plant and in general only the DEHP is worthy of note although its percentage transfer remains very low even in an experiment designed to maximize this.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of Chromatography A website: http://www.sciencedirect.com/science/article/pii/S0021967305003249
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Anjou Recherche (FRANCE)
Université de Toulouse > Ecole Nationale de Formation Agronomique - ENFA
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Recherche Agronomique - INRA
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Deposited By: Claire VIALLE

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