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Increase in Tomato Locule Number Is Controlled by Two Single-Nucleotide Polymorphisms Located

Munos, Stéphane and Ranc, Nicolas and Botton, Emmanuel and Berard, Aurélie and Rolland, Sophie and Duffe, Philippe and Carretero, Yolande and Le paslier, Marie-Christine and Delalande, Corinne and Bouzayen, Mondher and Brunel, Dominique and Causse, Mathilde Increase in Tomato Locule Number Is Controlled by Two Single-Nucleotide Polymorphisms Located. (2011) Plant Physiology, vol. 156 (n° 4). pp. 2244-2254. ISSN 0032-0889

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Official URL: http://dx.doi.org/10.1104/pp.111.173997

Abstract

In tomato (Solanum lycopersicum) fruit, the number of locules (cavities containing seeds that are derived from carpels) varies from two to up to 10 or more. Locule number affects fruit shape and size and is controlled by several quantitative trait loci(QTLs). The large majority of the phenotypic variation is explained by two of these QTLs, fasciated (fas) and locule number (lc), that interact epistatically with one another. FAS has been cloned, and mutations in the gene are described as key factors leading to the increase in fruit size in modern varieties. Here, we report the map-based cloning of lc. The lc QTL includes a 1,600-bp region that is located 1,080 bp from the 3# end of WUSCHEL, which encodes a homeodomain protein that regulates stem cell fate in plants. The molecular evolution of lc showed a reduction of diversity in cultivated accessions with the exception of two single-nucleotide polymorphisms. These two single-nucleotide polymorphisms were shown to be responsible for the increase in locule number. An evolutionary model of locule number is proposed herein, suggesting that the fas mutation appeared after the mutation in the lc locus to confer the extreme high-locule-number phenotype.

Item Type:Article
Additional Information:Thanks to American Society of Plant Biologists editor. The definitive version is available at http://my.aspb.org. The original PDF of the article can be found at Plant Physiology website : http://www.plantphysiol.org
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Université de Rennes 1 (FRANCE)
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Deposited By: Brigitte LAFFORGUE
Deposited On:21 Sep 2011 13:26

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