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Voltammetric microsensor using PEDOT-modified gold electrode for the simultaneous assay of ascorbic and uric acids

Sekli-Belaidi, Fadhila and Temple-Boyer, Pierre and Gros, Pierre Voltammetric microsensor using PEDOT-modified gold electrode for the simultaneous assay of ascorbic and uric acids. (2010) Journal of Electroanalytical Chemistry, vol. 647 (n° 2). pp.159-168. ISSN 1572-6657

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Official URL: http://dx.doi.org/10.1016/j.jelechem.2010.06.007

Abstract

A voltammetric microsensor has been developed for the simultaneous assay of ascorbic (AA) and uric (UA) acids in aqueous solution. The electrode surface has been modified by means of electropolymerized conductive poly(3,4-ethylenedioxythiophene) PEDOT organic films. The electrocatalytic activity of the interface was dependent on the electropolymerization parameters inducing change in the structure and the morphology of the resulting polymer. The PEDOT thickness was optimized in order to maximize the peak potential separation between both acids oxidation to more than 400 mV. By using differential pulse voltammetry (DPV), the sensitivity of the microsensor was 0.87 µA µM-1 cm-2 and 4.05 µA µM-1 cm-2 for AA and UA respectively. The later was sensible to the presence of AA in the mixture, making evidence of the catalytic mechanism of UA regeneration. The calibration curves were linear in the concentration range 5.0– 300 µmol L-1 for AA and 2.0–600 µmol L-1 for UA. The detection limits were 2.5 µmol L-1 and 1.5 µmol L-1 respectively. The sensor response was unmodified in the presence of the major electroactive biomarkers. The application of the PEDOT modified microsensor to the analysis of human blood serum was evaluated.

Item Type:Article
Additional Information:Thanks to Elsevier editor . The definitive version is available at http://dx.doi.org/10.1016/j.jelechem.2010.06.007
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
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Deposited By:David Evrard

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