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A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus

Riffault , Sabine and Meyer, Gilles and Deplanche, Martine and Dubuquoy, Catherine and Durand, Guillaume and Soulestin , Marion and Castagné, Nathalie and Bernard, Julie and Bernadet, Philippe and Dubosclard, Virginie and Bernex, Florence and Petit-Camurdan, Agnès and Deville, Sébastien and Schwartz-Cornil, Isabelle and Eléouët, Jean-François A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus. (2010) Vaccine, vol. 28 (21). pp. 3722-3734. ISSN 0264-410X

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Official URL: http://dx.doi.org/DOI:10.1016/j.vaccine.2010.03.008

Abstract

Human and bovine respiratory syncytial viruses (HRSV and BRSV) are two closely related, worldwide prevalent viruses that are the leading cause of severe airway disease in children and calves, respectively. Efficacy of commercial bovine vaccines needs improvement and no human vaccine is licensed yet. We reported that nasal vaccination with the HRSV nucleoprotein produced as recombinant ringshaped nanoparticles (NSRS) protects mice against a viral challenge with HRSV. The aim of this work was to evaluate this new vaccine that uses a conserved viral antigen, in calves, natural hosts for BRSV. Calves, free of colostral or natural anti-BRSV antibodies, were vaccinated with NSRS either intramuscularly, or both intramuscularly and intranasally using MontanideTM ISA71 and IMS4132 as adjuvants and challenged with BRSV. All vaccinated calves developed anti-N antibodies in blood and nasal secretions and N-specific cellular immunity in local lymph nodes. Clinical monitoring post-challenge demonstrated moderate respiratory pathology with local lung tissue consolidations for the non vaccinated calves that were significantly reduced in the vaccinated calves. Vaccinated calves had lower viral loads than the nonvaccinated control calves. Thus NSRS vaccination in calves provided cross-protective immunity against BRSV infection without adverse inflammatory reaction.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com. The original PDF of the article can be found at : http://bit.ly/jbL6ny
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Ecole Nationale Vétérinaire d'Alfort - ENVA (FRANCE)
Laboratory name:
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Deposited By: SYLVIE OUDDANE

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