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Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays

Leberre, Véronique and Baranowski , Eric and Deplanche, Martine and Trouilh , Lidwine and François, Jean Marie Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays. (2008) Journal of Virological Methods, vol. 148 . pp. 271-276. ISSN 0166-0934

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Official URL: http://dx.doi.org/10.1016/j.jviromet.2007.10.026

Abstract

Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis. With respect to a discrimination analysis, difference as small as one nucleotide base can be distinguished using oligonucleotide-basedmicroarrays. However, this degree of specificity is dependent on several parameters, including the size of the oligoprobes and the sequence context of the probes (e.g. local melting temperature), hybridization conditions and to some extent the chemistry of the glass slides onto which the probes are deposited. Using bovine respiratory syncytial virus (BRSV) as a model study, an oligonucleotide-based microarray approach was developed to measure the relative abundance of a particular single nucleotide variant within mixed BRSV populations. Using this technology, we show that it is possible to discriminate at a rate of 1%, minority variants in a BRSV population.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of Virological Methods : http://bit.ly/lITn6Q
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
French research institutions > Institut National de la Recherche en Informatique et en Automatique - INRIA
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA
Other partners > Platform Toulouse Genopole (FRANCE)
Laboratory name:
Statistics:download
Deposited By: SYLVIE OUDDANE

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