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Optimising DNA binding to carbon nanotubes by non-covalent methods

Sanz, Vanessa and Borowiak-Palen, Ewa and Lukanov, Petar and Galibert, Anne Marie and Flahaut, Emmanuel and Coley, Helen M. and Silva, S. Ravi P. and McFadden, Johnjoe Optimising DNA binding to carbon nanotubes by non-covalent methods. (2011) Carbon, vol. 49 (n° 5). pp. 1775-1781. ISSN 0008-6223

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Official URL: http://dx.doi.org/10.1016/j.carbon.2010.12.064

Abstract

The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors. To achieve this goal, carbon nanotubes have to be functionalized to become compatible with aqueous media and to bind the genetic material. To establish the best conditions for plasmid DNA binding, we compare the dispersion properties of single-, double- and multi-walled carbon nanotubes (SWCNTs, DWCNTs and MWCNTs, respectively) functionalized with a variety of surfactants by non-covalent attachment. The DNA binding properties of the functionalized carbon nanotubes were studied and compared by electrophoresis. Furthermore, a bilayer functionalization method for DNA binding on SWCNTs was developed that utilized RNA-wrapping to solubilize the nanotubes and cationic polymers as a bridge between nanotubes and DNA.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Carbon website : http://www.sciencedirect.com/science/journal/00086223
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > Szczecin University of Technology (POLAND)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > University of Surrey (UNITED KINGDOM)
Laboratory name:
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Deposited By: Leila Abdelouhab
Deposited On:17 May 2011 11:19

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