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The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression

Pinton, Philippe and Nougayrède, Jean-Philippe and Del Rio, Juan-Carlos and Moreno, Carolina and Marin, Daniela E. and Ferrier, Laurent and Bracarense, Ana-Paula and Kolf-Clauw, Martine and Oswald, Isabelle P The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression. (2009) Toxicology and Applied Pharmacology, vol. 237 (n° 1). pp. 41-48. ISSN 0041-008X

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Official URL: http://dx.doi.org/10.1016/j.taap.2009.03.003

Abstract

The gastrointestinal tract represents the first barrier against food contaminants as well as the first target for these toxicants. Deoxynivalenol (DON) is a mycotoxin that commonly contaminates cereals and causes various toxicological effects. Through consumption of contaminated cereals and cereal products, human and pigs are exposed to this mycotoxin. Using in vitro, ex vivo, and in vivo approaches, we investigated the effects of DON on the intestinal epithelium. We demonstrated that, in intestinal epithelial cell lines from porcine (IPEC-1) or human (Caco-2) origin, DON decreases trans-epithelial electric resistance (TEER) and increases in a time and dose-dependent manner the paracellular permeability to 4 kDa dextran and to pathogenic Escherichia Coli across intestinal cell monolayers. In pig explants treated with DON, we also observed an increased permeability of intestinal tissue. These alterations of barrier function were associated with a specific reduction in the expression of claudins, which was also seen in vivo in the jejunum of piglets exposed to DON-contaminated feed. In conclusion, DON alters claudin expression and decreases the barrier function of the intestinal epithelium. Considering that high levels of DON may be present in food or feed, consumption of DON-contaminated food/feed may induce intestinal damage and has consequences for human and animal health.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article is available at http://www.sciencedirect.com/science/journal/0041008X
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
Université de Toulouse > Ecole d'Ingénieurs de Purpan
French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Universidade Estadual de Londrina (BRASIL)
Laboratory name:
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Deposited By: martine kolf-clauw
Deposited On:08 Dec 2010 15:03

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