OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Guinea pig-adapted foot-and-mouth disease virus with altered receptor recognition can productively infect a natural host

Núñez, José I. and Molina, Nicolas and Baranowski, Eric and Domingo, Esteban and Clark, Stuart and Burman, Alison and Berryman, Stephen and Jackson, Terry and Sobrino, Francisco Guinea pig-adapted foot-and-mouth disease virus with altered receptor recognition can productively infect a natural host. (2007) Journal of Virology, vol. 81 (n° 16). pp. 8497-8506. ISSN 0022-538X

[img](Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
4Mb

Official URL: http://dx.doi.org/10.1128/JVI.00340-07

Abstract

We report that adaptation to infect the guinea pig did not modify the capacity of foot-and-mouth disease virus (FMDV) to kill suckling mice and to cause an acute and transmissible disease in the pig, an important natural host for this pathogen. Adaptive amino acid replacements (I(248)-->T in 2C, Q(44)-->R in 3A, and L(147)-->P in VP1), selected upon serial passages of a type C FMDV isolated from swine (biological clone C-S8c1) in the guinea pig, were maintained after virus multiplication in swine and suckling mice. However, the adaptive replacement L(147)-->P, next to the integrin-binding RGD motif at the GH loop in VP1, abolished growth of the virus in different established cell lines and modified its antigenicity. In contrast, primary bovine thyroid cell cultures could be productively infected by viruses with replacement L(147)-->P, and this infection was inhibited by antibodies to alphavbeta6 and by an FMDV-derived RGD-containing peptide, suggesting that integrin alphavbeta6 may be used as a receptor for these mutants in the animal (porcine, guinea pig, and suckling mice) host. Substitution T(248)-->N in 2C was not detectable in C-S8c1 but was present in a low proportion of the guinea pig-adapted virus. This substitution became rapidly dominant in the viral population after the reintroduction of the guinea pig-adapted virus into pigs. These observations illustrate how the appearance of minority variant viruses in an unnatural host can result in the dominance of these viruses on reinfection of the original host species.

Item Type:Article
Additional Information:Thanks to the American Society for Microbiology editor. The definitive version is available at http://jvi.asm.org/cgi/reprint/81/16/8497
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Institute for Animal Health (UNITED KINGDOM)
Other partners > Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (SPAIN)
Other partners > Universidad Autonoma de Madrid (SPAIN)
Laboratory name:
Statistics:download
Deposited By:Eric Baranowski

Repository Staff Only: item control page