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Characteristics of the tomato chromoplast revealed by proteomic analysis

Barsan, Cristina and Sanchez-Bel, Paloma and Rombaldi, César and Egea, Isabel and Rossignol, Michel and Kuntz, Marcel and Zouine, Mohamed and Latché, Alain and Bouzayen, Mondher and Pech, Jean-Claude Characteristics of the tomato chromoplast revealed by proteomic analysis. (2010) Journal of Experimental Botany, vol.61 (n°9). pp. 2413-2431.

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Official URL: http://jxb.oxfordjournals.org/content/early/2010/04/02/jxb.erq070.abstract

Abstract

Chromoplasts are non-photosynthetic specialized plastids that are important in ripening tomato fruit (Solanum lycopersicum) since, among other functions, they are the site of accumulation of coloured compounds. Analysis of the proteome of red fruit chromoplasts revealed the presence of 988 proteins corresponding to 802 Arabidopsis unigenes, among which 209 had not been listed so far in plastidial databanks. These data revealed several features of the chromoplast. Proteins of lipid metabolism and trafficking were well represented, including all the proteins of the lipoxygenase pathway required for the synthesis of lipid-derived aroma volatiles. Proteins involved in starch synthesis co-existed with several starch-degrading proteins and starch excess proteins. Chromoplasts lacked proteins of the chlorophyll biosynthesis branch and contained proteins involved in chlorophyll degradation. None of the proteins involved in the thylakoid transport machinery were discovered. Surprisingly, chromoplasts contain the entire set of Calvin cycle proteins including Rubisco, as well as the oxidative pentose phosphate pathway (OxPPP). The present proteomic analysis, combined with available physiological data, provides new insights into the metabolic characteristics of the tomato chromoplast and enriches our knowledge of non-photosynthetic plastids.

Item Type:Article
Additional Information:Thanks to Oxford Journals publisher. The definitive version is available at http://jxb.oxfordjournals.org/content/early/2010/04/02/jxb.erq070.abstract
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Universidade Federal de Pelotas - UFPel (BRAZIL)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Commissariat à l'Energie Atomique et aux énergies alternatives - CEA
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Université Joseph Fourier Grenoble 1 - UJF (FRANCE)
Université de Toulouse > Ecole Nationale de Formation Agronomique - ENFA
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
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Deposited By:Brigitte LAFFORGUE

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