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Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

Tramuta, C. and Robino, P. and Oswald, Eric and Nebbia, P. Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis. (2008) Veterinary Research Communications, vol. 32 (n° 1). pp. 1-5. ISSN 0165-7380

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Official URL: http://www.springerlink.com/content/v469486w53x55x8k

Abstract

A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the α1, α2, β, γ1, γ2/θ, κ, ɛ, ζ, and ι intimin variants.

Item Type:Article
Additional Information:Thanks to Kluwer Springer. The original publication is available at www.springerlink.com Veterinary Research Communications website : http://www.springerlink.com/content/103009
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Università degli studi di Torino - UNITO (ITALY)
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
Laboratory name:
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Deposited By: Mathieu Andro
Deposited On:20 May 2008 15:39

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