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Meat processing and colon carcinogenesis: Cooked, nitrite-treated and oxidized high-heme cured meat promotes mucin depleted foci in rats

Santarelli, Raphaëlle L. and Vendeuvre, Jean-Luc and Naud, Nathalie and Taché, Sylviane and Guéraud, Françoise and Viau, Michelle and Genot, Claude and Corpet, Denis E. and Pierre, Fabrice Meat processing and colon carcinogenesis: Cooked, nitrite-treated and oxidized high-heme cured meat promotes mucin depleted foci in rats. (2010) Cancer Prevention Research, vol. 3 (n° 7). pp. 852-864. ISSN 1940-6207

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Official URL: http://dx.doi.org/10.1158/1940-6207.CAPR-09-0160

Abstract

Processed meat intake is associated with colorectal cancer risk, but no experimental study supports the epidemiologic evidence. To study the effect of meat processing on carcinogenesis promotion, we first did a 14-day study with 16 models of cured meat. Studied factors, in a 2 × 2 × 2 × 2 design, were muscle color (a proxy for heme level), processing temperature, added nitrite, and packaging. Fischer 344 rats were fed these 16 diets, and we evaluated fecal and urinary fat oxidation and cytotoxicity, three biomarkers of heme-induced carcinogenesis promotion. A principal component analysis allowed for selection of four cured meats for inclusion into a promotion study. These selected diets were given for 100 days to rats pretreated with 1,2-dimethylhydrazine. Colons were scored for preneoplastic lesions: aberrant crypt foci (ACF) and mucin-depleted foci (MDF). Cured meat diets significantly increased the number of ACF/colon compared with a no-meat control diet (P = 0.002). Only the cooked nitrite-treated and oxidized high heme meat significantly increased the fecal level of apparent total N-nitroso compounds (ATNC) and the number of MDF per colon compared with the no-meat control diet (P < 0.05). This nitrite-treated and oxidized cured meat specifically increased the MDF number compared with similar non nitrite-treated meat (P = 0.03) and with similar non oxidized meat (P = 0.004). Thus, a model cured meat, similar to ham stored aerobically, increased the number of preneoplastic lesions, which suggests colon carcinogenesis promotion. Nitrite treatment and oxidation increased this promoting effect, which was linked with increased fecal ATNC level. This study could lead to process modifications to make non promoting processed meat.

Item Type:Article
Additional Information:Thanks to the American Association for Cancer Research editor. The original article can be found at http://cancerpreventionresearch.aacrjournals.org/
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Institut du Porc - IFIP (FRANCE)
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
Laboratory name:
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Deposited By: Denis CORPET
Deposited On:29 Jul 2010 12:42

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