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In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome

Deplanche, Martine and Lemaire, Mylène and Mirandette, Carole and Bonnet, Marion and Schelcher, François and Meyer, Gilles In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome. (2007) Journal of General Virology, vol. 88 (n°4). pp. 1260-1265. ISSN 0022-1317

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Official URL: http://dx.doi.org/10.1099/vir.0.82668-0

Abstract

We analyzed the genetic evolution of bovine respiratory syncytial virus (BRSV) isolate W2-00131, from its isolation in bovine turbinate (BT) cells to its inoculation in calves. Results showed that the BRSV genomic region encoding the highly variable glycoprotein G remains genetically stable after virus isolation and over 10 serial infections in BT cells, as well as following experimental inoculation in calves. This remarkable genetic stability led us to examine the mutant spectrum of several populations derived from this field isolate. Sequence analysis of molecular clones revealed an important genetic heterogeneity in G coding region of each population, with mutation frequencies ranging from 6.8 to 10.1 10-4 substitutions/nucleotide. The non-synonymous mutations of the mutant spectrum mapped preferentially within the two variable antigenic regions of the ectodomain or close to the highly conserved domain. These results suggest that RSV populations may evolve as complex and dynamic mutant swarms, despite apparent genetic stability.

Item Type:Article
Additional Information:Thanks to the Society for General Microbiology. The definitive version is available at http://vir.sgmjournals.org/. The original PDF of the article can be found at Journal of General Virology website: http://vir.sgmjournals.org/cgi/reprint/88/4/1260.pdf
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
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Deposited By: Gilles Meyer
Deposited On:28 Jul 2010 09:53

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