Akraim, Fowad and Nicot, Marie-Claude and Weill, P. and Enjalbert, Francis Effects of preconditioning and extrusion of linseed on the ruminal biohydrogenation of fatty acids. 2. In vitro and in situ studies. (2006) Animal Research, vol. 5 (n° 4). pp. 261-271. ISSN 1627-3583
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Official URL: http://dx.doi.org/10.1051/animres:2006023
The extent and/or intermediates of ruminal biohydrogenation (BH) of fatty acids (FA) were investigated in vitro and in situ using a raw, pre-conditioned or extruded blend of linseed and wheat bran (70:30). The duration of in vitro incubations were 2, 4, 8, 16 and 24 h, with 5 replicates. In situ studies used 3 dry ruminally fistulated Holstein cows in a 3 × 3 Latin square design, with 3 weeks adaptation to the linseed form. The diet contained 20% (DM basis) of the linseed based blend. The duration of in situ incubations were 2, 4, 8, 16, 24 and 48 h. BH was much slower in situ than in vitro, resulting in a much lower effective disappearance of C18:2 and C18:3. Moreover, the in situ technique suggested that the technological pre-treatment of linseed did not affect C18:2 and C18:3 rate of BH, whereas reduced rates were observed in vitro. After 8 h of in vitro incubation and onwards, proportions of cis-9,trans-11C18:2 were the highest with extruded linseed. The BH of FA from linseed resulted in the appearance of great proportions of trans-10+11 to trans-16C18:1 intermediates. Extrusion increased the proportions of trans-10+11C18:1 both in vitro and in situ and proportions or trans-C18:1 were higher in situ than in vitro. Compared to previous in vivo results with the same material, the in situ method provided poor estimates of BH rates and intermediates.
|Additional Information:||Thanks to EDP Sciences editor. The definitive version is available at http://animres.edpsciences.org/|
|Audience (journal):||International peer-reviewed journal|
|Institution:||Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT|
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Other partners > VALOREX (FRANCE)
|Deposited By:||Annabelle Troegeler|
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