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AFM imaging of functionalized carbon nanotubes on biological membranes

Lamprecht, Constanze and Liashkovich, I. and Neves, Vera and Danzberger, Jürgen and Heister, Elena and Rangl, M. and Coley, Helen M. and McFadden, Johnjoe and Flahaut, Emmanuel and Gruber, Hermann J. and Hinterdorfer, Peter and Kienberger, Ferry and Ebner, Andreas AFM imaging of functionalized carbon nanotubes on biological membranes. (2009) Nanotechnology, vol. 20 (n° 43). ISSN 1361-6528

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Official URL: http://dx.doi.org/10.1088/0957-4484/20/43/434001

Abstract

Multifunctional carbon nanotubes are promising for biomedical applications as their nano-size, together with their physical stability, gives access into the cell and various cellular compartments including the nucleus. However, the direct and label-free detection of carbon nanotube uptake into cells is a challenging task. The atomic force microscope (AFM) is capable of resolving details of cellular surfaces at the nanometer scale and thus allows following of the docking of carbon nanotubes to biological membranes. Here we present topographical AFM images of non-covalently functionalized single walled (SWNT) and double walled carbon nanotubes (DWNT) immobilized on different biological membranes, such as plasma membranes and nuclear envelopes, as well as on a monolayer of avidin molecules. We were able to visualize DWNT on the nuclear membrane while at the same time resolving individual nuclear pore complexes. Furthermore, we succeeded in localizing individual SWNT at the border of incubated cells and in identifying bundles of DWNT on cell surfaces by AFM imaging.

Item Type:Article
Additional Information:Thanks to Institute of Physics editor. The definitive version is available at www.iop.org/ The original PDF of the article can be found at Nanotechnology website : http://iopscience.iop.org/0957-4484/20/43/434001/
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Agilent Technologies (USA)
Other partners > Linz Johannes Kepler University (AUSTRIA)
Other partners > University of Surrey (UNITED KINGDOM)
Other partners > Westfälische Wilhelms-University of Münster (GERMANY)
Laboratory name:
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Deposited By: Nihad Lahdifi

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