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Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test

El Hajjouji, Houda and Pinelli, Eric and Guiresse, Maritxu and Merlina, Georges and Revel, Jean-Claude and Hafidi, Mohamed Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test. (2007) Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 6 (n° 1-2). pp. 25-31. ISSN 1383-5718

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Official URL: http://dx.doi.org/10.1016/j.mrgentox.2007.05.015

Abstract

The present study concerns the genotoxicity of olive mill waste water (OMWW) generated in mills producing olive oil in Morocco. The Vicia faba micronucleus test was used to evaluate the genotoxicity of OMWW and the six major phenolic compounds identified by HPLC in this effluent. Five dilutions of OMWW were tested: 0.1, 1, 5, 10 and 20%. Maleic hydrazide was used as a positive control. The results showed that OMWW was genotoxic at 10% dilution. In order to investigate the components involved in this genotoxicity, the six major phenols present in this effluent, oleuropein, gallic acid, 4-hydroxyphenyl acetic acid, caffeic acid, paracoumaric acid and veratric acid, were studied at concentrations corresponding to the genotoxic concentration of the OMWW itself. Two phenols, gallic acid and oleuropein induced a significant increase in micronucleus frequency in Vicia faba; the four other phenols had no significant genotoxic effect. These results suggest that under the experimental conditions of our assay, OMWW genotoxicity was associated with gallic acid and oleuropein.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Mutation Research - Genetic Toxicology and Environmental Mutagenesis website : http://www.sciencedirect.com/science/journal/13835718
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > University of Cadi Ayyad - UCAM (MOROCCO)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
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Deposited By: Florence Amor

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