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Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots

Pourrut, Bertrand and Perchet, Geoffrey and Silvestre, Jérôme and Cecchi, Marie and Guiresse, Maritxu and Pinelli, Eric Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots. (2008) Journal of Plant Physiology, vol. 165 (n° 6). ISSN 0176-1617

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Official URL: http://dx.doi.org/10.1016/j.jplph.2007.07.016

Abstract

The mechanism of oxidative burst induced by lead in Vicia faba excised roots was investigated by luminol-dependent chemiluminescence. Results showed that lead triggered a rapid and dose-dependent increase in chemiluminescence production. In this study, specific inhibitors of putative reactive oxygen species (ROS) sources were used to determine the mechanism of lead-induced ROS generation. This generation was sensitive to dephenylene iodonium (DPI), quinacrine and imidazole, some inhibitors of the NADPH-oxidase and not inhibited by other putative ROS sources inhibitors. Data reported in this work clearly demonstrated the pivotal role of NADPH-oxidase-like enzyme in early steps of lead-induced oxidative burst. To investigate the respective implication of calmodulin and protein kinase (PK) in leadinduced NADPH-oxidase activation, excised roots were treated with the calmodulin inhibitor W7 or with the PK inhibitor staurosporine. The chemiluminescence generation inhibition by these inhibitors illustrated the role of PK in lead-induced NADPH-oxidase activation and revealed a calmodulin-dependent step. Using the calcium entry blocker La 3+ or different concentrations of calcium in the extracellular medium, our data highlighted the implication of Ca 2+ channel in leadinduced oxidative burst.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of Plant Physiology website : http://www.sciencedirect.com/science/journal/01761617
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
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Deposited By: Florence Amor

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