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Response of Vicia faba L. to metal toxicity on mine tailing substrate:Geochemical and morphological changes in leaf and root

Probst, Anne and Liu, Hongyu and Fanjul, Marjorie and Liao, Bo-han and Hollande, Etienne Response of Vicia faba L. to metal toxicity on mine tailing substrate:Geochemical and morphological changes in leaf and root. (2009) Environmental and Experimental Botany, vol. 66 (n° 2). pp. 297-308. ISSN 0098-8472

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Official URL: http://dx.doi.org/10.1016/j.envexpbot.2009.02.003

Abstract

Vicia faba L. seeds were grown in a pot experiment on soil, mine tailings, and a mixture of both to mimic field situations in cultivated contaminated areas near mining sites. Metals in the substrates and their translocation in root, stem and leaf tissues were investigated, including morphological responses of plants growing on mine tailings. Metal concentration � and generally bioaccumulation � was in the order: roots > leaves > stems, except Pb and Cd. Translocation was most significant for Zn and Cd, but limited for Pb. Metal concentration in root and leaf was not proportional to that in the substrates, unexpectedly the minimum being observed in the mixed substrate whilst plant growth was retarded by 20% (38% on tailings). Calcium, pH, organic matter and phosphorus were the main influencing factors for metal translocation. The ultrastructure of V. faba L. cells changed a lot in the mine tailings group: root cell walls were thickened with electron dense Pb, Zn and C particles; in chloroplasts, the number of plastoglobuli increased, whereas the thylakoids were swollen and their number decreased in grana. Finally, needle-shaped crystalline concretions made of Ca and P, with Zn content, were formed in the apoplast of the plants. The stratagems of V. faba L. undergoing high concentrations of toxic metals in carbonate substrate, suggest root cell wall thickening to decrease uptake of toxic metals, a possible control of metal transport from roots to leaves by synthesizing phytochelators�toxic metal complexes, and finally a control of exceeded Ca and metal concentration in leaves by crystal P formation as ultimate response to stress defense. The geochemical factors influencing metal availability, guaranty a reduction of metal content in plant growing on mixed tailing/soil substrate as far as carbonate are not completely dissolved.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Environmental and Experimental Botany website : http://www.elsevier.com/wps/find/journaldescription.cws_home/267/description#description
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Central South University of Forestry and Technology - CSUFT (CHINA)
Other partners > Hunan University (CHINA)
Laboratory name:
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Deposited By:Florence Amor

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