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Development of a pig jejunal explant culture for studying the gastrointestinal toxicity of the mycotoxin deoxynivalenol: histopathological analysis

Kolf-Clauw, Martine and Castellote, Jessie and Joly, Benjamin and Bourges-Abella, Nathalie and Raymond-Letron, Isabelle and Pinton, Philippe and Oswald, Isabelle P Development of a pig jejunal explant culture for studying the gastrointestinal toxicity of the mycotoxin deoxynivalenol: histopathological analysis. (2009) Toxicology in Vitro, vol. 23 . pp. 1580-1584. ISSN 0887-2333

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Official URL: http://www.sciencedirect.com/science/journal/08872333

Abstract

The digestive tract is a target for the mycotoxin deoxynivalenol (DON), a major cereals grain contaminant of public health concern in Europe and North America. Pig, the most sensitive species to DON toxicity, can be regarded as the most relevant animal model for studying the intestinal effects of DON. A pig jejunal explants culture was developed to assess short-term effects of DON. In a first step, jejunal explants from 9-13 week-old and from 4-5 week-old pigs were cultured in vitro for up to 8 hours. Explants from younger animals were better preserved after 8 hours, as assessed by morphological scores and by villi lengths. In a second step, dose-related alterations of the jejunal tissue were observed, including shortened and coalescent villi, lysis of enterocytes, oedema. After 4h of DON exposure of explants from 4-5 week-old pigs, a no-effect concentration level of 1 µM was estimated (corresponding to diet contaminated with 0.3 mg DON/kg) based on morphological scores, and of 0.2 µM based on villi lengths. In conclusion, our data indicate that pig intestinal explants represent a relevant and sensitive model to investigate the effects of food contaminants.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Toxicology in Vitro website : http://www.sciencedirect.com/science/journal/08872333
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
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Deposited By: martine kolf-clauw

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