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Minimum Antibiotic Levels for Selecting a Resistance Plasmid in a Gnotobiotic Animal Model

Corpet, Denis E. and Lumeau, Solange and Corpet, Florence Minimum Antibiotic Levels for Selecting a Resistance Plasmid in a Gnotobiotic Animal Model. (1989) Antimicrobial Agents and Chemotherapy, vol. 3 (n° 4). pp. 535-540. ISSN 0066-4804

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Abstract

The minimum antibiotic concentrations for selecting an R plasmid in vivo were determined in germfree rates colonized by two isogenic strains of Escherichia coli, one of which carried an R plasmid. Seventy groups of three gnotobiotic mice were given low doses of ampicillin, colistin, flumequin, gentamicin, tetracycline, or streptomycin via drinking water for 2 weeks. The equilibrium between susceptible and resistant populations of bacteria was monitored daily in feces and compared with that of control mice given pure water. This model yielded reproducible data, and dose and response were strongly correlated. The minimum selecting doses ranged from 0.9 te 12.8 µg/ml of water, depending on the antibiotic and the R plasmid. The use of mathematical models and complementary in vitro experiments accounted for the effect of the low antibiotic levels.

Item Type:Article
Additional Information:Journal website : http://aac.asm.org/
Audience (journal):International peer-reviewed journal
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Institution: Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
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Deposited By: Denis CORPET
Deposited On:04 Dec 2009 11:07

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