Corpet, Denis E. and Nicolas, Jean-Louis Antagonistic Effect of Intestinal Bacteria from the Microflora of Holoxenic (Conventional) Piglets, Against Clostridium Perfringens in the Digestive Tract of Gnotoxenic Mice and Gnotoxenic Piglets. (1979) In: Clinical and experimental gnotobiotics : proceedings of the VIth International Symposium on Gnotobiology, Ulm (Fed. Rep. of Germany), June 6-10, 1978 .
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Antagonistic effect of piglet microflora against Clostridium perfringens was studied in germfree mice, to isolate bacterial strains responsible for this colonization resistance. The 1:100 dilution of the feces of a 2 day-old conventional piglet, given per os to germfree mice already harboring C. perfringens, led to the elimination of C. perfringens. From this piglet flora, 8 bacterial strains were selected, belonging to the genera Bacteroides, Clostridium, Eubacterium, Bifidobacterium, Lactobacillus and a strain belonging to the class of Mollicutes. When the 8 strains were given to germfree mice 3 days after C. perfringens inoculation, they led to rapid elimination of C. perfringens from feces. Sixteen other mixtures of 2 to 7 strains were similarly tested, but none was able to fully antagonize C. perfringens. When the 8 strains were given per os to germfree piglets after C. perfringens inoculation, they led to the rapid elimination of C. perfringens from pig feces, and to a quick recovery from diarrhea. This study led to the identification of a simplified fraction of gut microflora, able to exert a barrier effect against C. perfringens comparable to the entire flora of the piglet. This study suggests that gnotoxenic mice can be a suitable model for simplifying the flora responsible for a given effect in another host, animal or human.
|Item Type:||Conference or Workshop Item (Paper)|
|Additional Information:||This article was published in Zentralblatt für Bakteriologie Mikrobiologie und Hygiene - suppl. 7 .Corpet D., Nicolas J.L "Antagonistic Effect of Intestinal Bacteria from the Microflora of Holoxenic (Conventional) Piglets, Against Clostridium Perfringens in the Digestive Tract of Gnotoxenic Mice and Gnotoxenic Piglets", Pages 169-172, Copyright Elsevier (1979) Thanks to Elsevier editor. Website : http://www.sciencedirect.com/science/journal/14384221|
|Audience (journal):||Special issue journal|
|Audience (conference):||International conference proceedings|
Microbiote - Gut flora - Fecal microflora - Antagonistic effect - Colonization resistance - Barrier effect - Isolator, bacteria - Clostridium perfringen - Bacteroides - Clostridium - Eubacterium - Bifidobacterium - Lactobacillus - Mollicutes - Microbial ecology - Germfree - Axenic,gnotoxenic - Mouse - Mice - Animal model - Pig - Swine - Piglet - Diarrhea
|Institution:||French research institutions > Institut National de la Recherche Agronomique - INRA|
|Deposited By:||Denis CORPET|
|Deposited On:||27 Nov 2009 14:01|
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