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Antibiotic residues and R-plasmid selection: are in vitro methods good models?

Corpet, Denis E. and Lumeau, Solange Antibiotic residues and R-plasmid selection: are in vitro methods good models? (1987) Zentralblatt für Bakteriologie Mikrobiologie und Hygiene - series A, 264 (1-2). pp. 178-184. ISSN 0176-6724

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Abstract

Three clones of E. coli, one of which was harbouring a tetracycline resistance plasmid were inoculated together into the stomach of axenic mice. Without antibiotic selective pressure, the R-Plasmid bearing strain became dominant in the faeces of mice, while the R-plasmid free strain was eliminated. When the R-plasmid bearing strain was given to mice 4 days after the inoculation with the R-plasmid free strain, it was repressed and remained at the stable level of 10(4.5) organisms per g of faeces. But a rapid spread of the R-plasmid was observed, tetracycline resistant bacteria become dominant within one day, and replace the tetracycline sensitive E. coli. The tetracycline resistance plasmid did not disadvantage the mediating strain in the gut, even in the absence of antibiotic pressure. In contrast Lebek and Egger (1983), studying the same strains in vitro, found that in a chemostat the plasmid bearing strain was overgrown by the plasmid free strain. These results strongly suggest that in vitro interactions between E. coli strains cannot be directly extrapolated to in vivo conditions. For the determination of the no-effect level of antibiotic residue on the selection of R-factor in the gut, studies should be made in vivo.

Item Type:Article
Additional Information:This article was published in Zentralblatt für Bakteriologie Mikrobiologie und Hygiene - series A, Vol 264, Corpet D., Lumeau S. Antibiotic residues and R-plasmid selection: are in vitro methods good models?, Pages 178-184, Copyright Elsevier (1987) Thanks to Elsevier editor. Website : http://www.sciencedirect.com/science/journal/14384221
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT
Laboratory name:
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Deposited By: Denis CORPET

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