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Comparative Evaluation of the Toxicity and Genotoxicity of Cadmium in Amphibian Larvae (Xenopus laevis and Pleurodeles waltl) Using the Comet Assay and the Micronucleus Test

Mouchet, Florence and Gauthier, Laury and Baudrimont, Magalie and Gonzalez, Patrice and Mailhes, Corinne and Ferrier, Vincent and Devaux, Alain Comparative Evaluation of the Toxicity and Genotoxicity of Cadmium in Amphibian Larvae (Xenopus laevis and Pleurodeles waltl) Using the Comet Assay and the Micronucleus Test. (2007) Environmental Toxicology, Vol.22 (N° 4). pp. 422-435. ISSN 1520-4081

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Official URL: http://dx.doi.org/10.1002/tox.20267

Abstract

The toxic and genotoxic potential of Cadmium (CdCl2) were evaluated by the micronucleus test (MNT) and comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that Cd is toxic to Xenopus larvae exposed from 2 to 50 mg/L and to Pleurodeles from 5 to 50 mg/L, depending on the nature of the water (reconstituted water containing mineral salts or mineral water MW (Volvic1)). The MNT results obtained in MW showed that Cd (2 mg/L) is genotoxic to Xenopus, whereas it was not genotoxic to Pleurodeles at all concentrations tested. The CA established that the genotoxicity of Cd to Xenopus and Pleurodeles larvae depends on the concentration, the exposure times, and the comet parameters (Tail DNA, ETM, OTM, and TL). The CA and MNT results were compared for their ability to detect genotoxic effects, considering the concentrations of Cd applied and the exposure time. The CA showed Cd to be genotoxic from the first day of exposure. In amphibians, the CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by Cd.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Université de Toulouse I-Sciences Sociales - UT1
French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Université de Toulouse II-Le Mirail - UTM
French research institutions > Institut National de la Recherche Agronomique - INRA
Other partners > Ecole Nationale des Travaux Publics de l'Etat - ENTPE (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > Université de Bordeaux 1 (FRANCE)
Laboratory name:
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Deposited By: Corinne MAILHES
Deposited On:02 Oct 2009 08:03

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