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Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (MutatoxR and Ames tests)

Mouchet, Florence and Gauthier, Laury and Mailhes, Corinne and Jourdain, M-J and Ferrier, Vincent and Triffault, G. and Devaux, Alain Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (MutatoxR and Ames tests). (2006) Science of the Total Environment, vol. 3 (n°1-3). pp. 232-246.

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Official URL: http://dx.doi.org/10.1016/j.scitotenv.2005.02.031

Abstract

The management of contaminated soils and wastes is a matter of considerable human concern. The present study evaluates the genotoxic potential of aqueous extracts of two soils (leachates) and of bottom ash resulting from municipal solid waste incineration (MSWIBA percolate), using amphibian larvae (Xenopus laevis). Soil A was contaminated by residues of solvents and metals and Soil B by polycyclic aromatic hydrocarbons and metals. MSWIBA was predominantly contaminated by metals. Two genotoxic endpoints were analysed in circulating erythrocytes taken from larvae: clastogenic and/or aneugenic effects (micronucleus induction) after 12 days of exposure and DNA-strand-breaking potency (comet assay) after 1 and 12 days of exposure. In addition, in vitro bacterial assays (MutatoxR and Ames tests) were carried out and the results were compared with those of the amphibian test. Physicochemical analyses were also taken into account. Results obtained with the amphibians established the genotoxicity of the aqueous extracts and the comet assay revealed that they were genotoxic from the first day of exposure. The latter test could thus be considered as a genotoxicity-screening tool. Although genotoxicity persisted after 12 days’ exposure, DNA damage decreased overall between days 1 and 12 in the MSWIBA percolate, in contrast to the soil leachates. Bacterial tests detected genotoxicity only for the leachate of soil A (Mutatox). The results confirm the ecotoxicological relevance of the amphibian model and underscore the importance of bioassays, as a complement to physicochemical data, for risk evaluation.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The original PDF of the article can be found at Science of the Total Environment website : http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V78-4FWS78D-3&_user=4373306&_coverDate=02%2F15%2F2006&_alid=1021539034&_rdoc=1&_fmt=high&_orig=search&_cdi=5836&_sort=r&_docanchor=&view=c&_ct=14&_acct=C000043979&_version=1&_urlVersion=0&_userid=4373306&md5=27d8d6bca6591700f7d8bac2255e00f7
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National des Etudes Spatiales - CNES
French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Ecole Nationale de l'Aviation Civile - ENAC
Other partners > Ecole Nationale des Travaux Publics de l'Etat - ENTPE (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Recherche Agronomique - INRA
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM
Université de Toulouse > Institut Supérieur de l'Aéronautique et de l'Espace - ISAE
Other partners > Thales (FRANCE)
Other partners > Telecom ParisTech (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Université de Toulouse > Université de Toulouse II-Le Mirail - UTM
Université de Toulouse > Université de Toulouse I-Sciences Sociales - UT1
Laboratory name:
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Deposited By: Corinne MAILHES

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