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Comparative Study of the Comet Assay and the Micronucleus Test in Amphibian Larvae (Xenopus laevis) Using Benzo(a)pyrene, Ethyl Methanesulfonate, and Methyl Methanesulfonate: Establishment of a Positive Control in the Amphibian Comet Assay

Mouchet, Florence and Gauthier, Laury and Mailhes, Corinne and Ferrier, Vincent and Devaux, Alain Comparative Study of the Comet Assay and the Micronucleus Test in Amphibian Larvae (Xenopus laevis) Using Benzo(a)pyrene, Ethyl Methanesulfonate, and Methyl Methanesulfonate: Establishment of a Positive Control in the Amphibian Comet Assay. (2005) Environmental Toxicology, vol. 2 (n°1). pp. 74-84. ISSN 1520-4081

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Official URL: http://dx.doi.org/10.1002/tox.20080

Abstract

The present investigation explored the potential use of the comet assay (CA) as a genotoxicity test in the amphibian Xenopus laevis and compared it with the French standard micronucleus test (MNT). Benzo[a]pyrene (B[a]P), methyl methanesulfonate (MMS), and ethyl methanesulfonate (EMS) were used as model compounds for assessing DNA damage. Damage levels were measured as DNA strand breaks after alkaline electrophoresis of nuclei isolated from larval amphibian erythrocytes using the CA in order to establish a positive control for further ecotoxicological investigations. The results led to the selection of MMS as a positive control on the basis of the higher sensitivity of Xenopus laevis to this compound. The CA and MNT were compared for their ability to detect DNA damage with the doses of chemical agents and exposure times applied. EMS and MMS were shown to increase micronucleus and DNA strand break formation in larval erythrocytes concurrently. However, B[a]P increased micronucleus formation but not that of DNA strand breaks. Time–dose experiments over 12 days of exposure suggest that the CA provides an earlier significant response to genotoxicants than does the MNT. In Xenopus the CA appears to be a sensitive and suitable method for detecting genotoxicity like that caused by EMS and MMS. It can be considered a genotoxicityscreening tool. The results for B[a]P show that both tests should be used in a complementary manner on Xenopus.

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National des Etudes Spatiales - CNES
French research institutions > Centre National de la Recherche Scientifique - CNRS
Université de Toulouse > Ecole Nationale de l'Aviation Civile - ENAC
Other partners > Ecole Nationale des Travaux Publics de l'Etat - ENTPE (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
French research institutions > Institut National de la Recherche Agronomique - INRA
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM
Université de Toulouse > Institut Supérieur de l'Aéronautique et de l'Espace - ISAE
Other partners > Rockwell Collins (FRANCE)
Other partners > Thales (FRANCE)
Other partners > Telecom ParisTech (FRANCE)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Université de Toulouse > Université de Toulouse II-Le Mirail - UTM
Université de Toulouse > Université de Toulouse I-Sciences Sociales - UT1
Other partners > Direction Générale de l’Aviation Civile - DGAC (FRANCE)
Laboratory name:
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Deposited By: Corinne MAILHES
Deposited On:23 Sep 2009 13:24

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