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Electrochemical microsensors for cutaneous surface analysis: Application to the determination of pH and the antioxidant properties of stratum corneum

Baur, J. and Comtat, Maurice and Gros, Pierre and Questel, Emmanuel and Ruffien-Ciszak, Audrey Electrochemical microsensors for cutaneous surface analysis: Application to the determination of pH and the antioxidant properties of stratum corneum. (2008) IRBM, Vol.29 (n°2-3). pp.162-170. ISSN 1959-0318

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Official URL: http://dx.doi.org/10.1016/j.rbmret.2007.11.020

Abstract

Potentiometry and cyclic voltammetry were proposed as simple, reliable and non invasive methods for the simultaneous determination of pH and antioxidant properties of skin. Experiments were performed with microelectrodes just deposited on skin surface without any gel or water added. pH was measured by means of the zero current potential of a tungsten W/WO3 sensor. A nerstian response was recorded in pH range 4 to 6 corresponding to the normal skin pH values. The global antioxidant capacity was deduced from the anodic charge passed during the plotting of cyclic voltammograms on platinum or gold microelectrodes. Comparing the half wave or peak potentials of these curves with those recorded for experiments performed in aqueous solution, the main hydrophilic antioxidants species were detected, i.e. ascorbic acid, uric acid and glutathione. This relatively easy-to-use analytical method made it possible to follow in real time the efficiency of topic treatment as well as to study the influence of oxidative stress

Item Type:Article
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Laboratoires Pierre Fabre (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
Laboratory name:
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Deposited By: Guylène Abadie

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