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Assessment of lead ecotoxicity in water using the amphibian larvae (Xenopus laevis) and preliminary study of its immobilization in meat and bone meal combustion residues

Mouchet, Florence and Cren, S. and Cunienq, C. and Deydier, Eric and Guilet , Richard and Gauthier, Laury Assessment of lead ecotoxicity in water using the amphibian larvae (Xenopus laevis) and preliminary study of its immobilization in meat and bone meal combustion residues. (2007) BioMetals, Vol.20 (n° 2 ). p.113-127. ISSN 0966-0844

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Official URL: http://dx.doi.org/0.1007/s10534-006-9019-x

Abstract

Lead (Pb) is a major chemical pollutant of the environment. It has been associated with human activities for the last 6000 years. Quite rightly, it remains a public health concern today. The present investigation evaluates the toxic potential of Pb in larvae of the toad Xenopus laevis after 12 days exposure in lab conditions. Acute toxicity, genotoxicity and Pb bioaccumulation were analyzed. The genotoxic effects were analyzed in the circulating blood from the levels of micronucleus induction according to the French standard micronucleus assay (AFNOR 2000 Association franc¸ aise de normalization. Norme NFT 90-325. Qualite´ de l’Eau. Evaluation de la ge´notoxicite´ au moyen de larves d’amphibien (Xenopus laevis, Pleurodeles waltl)). Lead bioaccumulation was analyzed in the liver of larvae at the end of exposure. Moreover, the toxic potential of lead, in aquatic media, was investigated in the presence of meat and bone meal combustion residues (MBMCR) known to be rich in phosphates and a potential immobiliser of lead. Previously, acute toxicity and genotoxicity of MBMCR alone were evaluated using Xenopus larvae. The results obtained in the present study demonstrated: (i) that lead is acutely toxic and genotoxic to amphibian larvae from 1 mg Pb/l and its bioaccumulation is significant in the liver of larvae from the lowest concentration of exposure (1 lg Pb/l), (ii) MBMCR were not acutely toxic nor genotoxic in Xenopus larvae, (iii) lead in presence of MBMCR induced inhibition or reduction of the toxic and genotoxic potential of lead in water at concentrations that do not exceed the capacity of MBMCR of Pb-binding (iv) Pb accumulation in larvae exposed to lead with MBMCR in water was lower than Pb-accumulation in larvae exposed to lead alone except at the concentration of 0.01 mg Pb/l suggesting complex mechanisms of MBMCR interaction in organisms. The results confirm the high toxicity and genotoxicity of lead in the aquatic compartment and suggest the potential utility of MBMCR for use in remediation.

Item Type:Article
Additional Information:You can find this article in http://www.springerlink.com/content/1040684p3p2812l1/
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
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Deposited By: Guylène Abadie

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