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Effect of carbon nanotubes on developing zebrafish (Danio Rerior) embryos

Cheng, Jinping and Flahaut, Emmanuel and Cheng, Shuk Han Effect of carbon nanotubes on developing zebrafish (Danio Rerior) embryos. (2007) Environmental Toxicology and Chemistry, vol. 26 (n° 4). pp. 708-716. ISSN 0730-7268

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Official URL: http://dx.doi.org/10.1897/06-272R.1

Abstract

The impact of carbon nanotubes (CNTs) on the aquatic environment was investigated by examining the properties of raw CNTs under several environmental conditions and using developing zebrafish (Danio rerio) embryos. The agglomerate size for single-walled CNTs (SWCNTs) was significantly larger at pH 11 or greater and was stable at temperatures from 4 to 40C and salinities from 0 to 30 ppt. Exposure to SWCNTs induced a significant hatching delay in zebrafish embryos between 52 to 72 h postfertilization (hpf ) at concentrations of greater than 120 mg/L, but 99% of the exposed embryos hatched by 75 hpf. Doublewalled CNTs also induced a hatching delay at concentrations of greater than 240 mg/L, but carbon black did not affect hatching at the concentrations tested. Molecular and cellular analysis showed that the embryonic development of the exposed embryos up to 96 hpf was not affected at SWCNT concentrations of up to 360 mg/L. Scanning-electron microscopic inspection showed that the size of the pores on the embryo chorion was nanoscaled and that the size of SWCNT agglomerates was microscaled or larger, indicating that the chorion of zebrafish embryos was an effective protective barrier to SWCNT agglomerates. The hatching delay observed in this study likely was induced by the Co and Ni catalysts used in the production of SWCNTs that remained at trace concentrations after purification. This study suggests that materials associated with raw SWCNTs (perhaps metal contaminants) have the potential to affect aquatic life when released into the aquatic environment.

Item Type:Article
Additional Information:Thanks to Society of Environmental Toxicology and Chemistry Press - SETAC editor. The definitive version is available at http://www.setacjournals.org The original PDF of the article can be found at Environmental Toxicology and Chemistry website : http://www.setacjournals.org/perlserv/?request=index-html
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Other partners > City University of Hong Kong (CHINA)
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
French research institutions > Centre National de la Recherche Scientifique - CNRS
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Deposited By: Celine Cabaup
Deposited On:18 Jun 2009 12:36

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