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Binding of pulmonary surfactant proteins to carbon nanotubes; potential for damage to lung immune defense mechanisms

Salvador-Morales, Carolina and Townsend, Paul and Flahaut, Emmanuel and Vénien-Bryan, Catherine and Vlandas, Alexis and Green, Malcolm L. H. and Sim, Robert B. Binding of pulmonary surfactant proteins to carbon nanotubes; potential for damage to lung immune defense mechanisms. (2007) Carbon, vol. 45 (n° 3). pp. 607-617. ISSN 0008-6223

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Official URL: http://dx.doi.org/10.1016/j.carbon.2006.10.011

Abstract

Potential pulmonary toxicity of carbon nanotubes is a research area that has received considerable attention. Surfactant proteins A and D (SP-A and SP-D) are collectin proteins that are secreted by airway epithelial cells in the lung. They play an important role in firstline defense against infection within the lung. The aim of this study was to investigate the interaction between carbon nanotubes and proteins contained in lung surfactant. By using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western Blotting, a novel technique of affinity chromatography based on carbon nanotube–Sepharose matrix [1] and electron microscopy data it was shown that SP-A and SP-D selectively bind to carbon nanotubes. The binding was Ca2+-ion dependent, and was variable between batches of nanotubes. It was therefore likely to be mediated by surface impurities or chemical modifications of the nanotubes. Chronic level exposure to carbon nanotubes may result in sequestration of SP-D and SP-A. Absence of these proteins in knockout mice leads to susceptibility to lung infection and emphysema.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Carbon website : http://www.sciencedirect.com/science/journal/00086223
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution: Université de Toulouse > Institut National Polytechnique de Toulouse - INPT
Université de Toulouse > Université Paul Sabatier-Toulouse III - UPS
Other partners > University of Oxford (UNITED KINGDOM)
French research institutions > Centre National de la Recherche Scientifique - CNRS
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Deposited By: Celine Cabaup
Deposited On:12 May 2009 08:58

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