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Ethanol triggers grape gene expression leading to anthocyanin accumulation during berry ripening

El-Kereamy, Ashraf and Chervin, Christian and Souquet, Jean-Marc and Moutounet, Michel and Monje, Marie-Carmen and Nepveu, Françoise and Mondies, Hélène and Ford, Christopher M and van Heeswijck, Robyn and Roustan, Jean-Paul Ethanol triggers grape gene expression leading to anthocyanin accumulation during berry ripening. (2002) Plant Science, 163 (3). 449-454. ISSN 0168-9452

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Official URL: https://doi.org/10.1016/S0168-9452(02)00142-5

Abstract

Recent studies have shown that low doses of ethanol stimulate the maturation of some fruits. The present work showed that spraying Cabernet Sauvignon grapes, with 5% ethanol at veraison enhances the anthocyanin accumulation. Veraison is the time when the berries turn from green to purple. HPLC analysis showed a marked increase in the total concentrations of the derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin from the fourth day after the ethanol treatment until harvest. This was not linked to a difference in berry weight in comparison to controls. Two distinct expression patterns were found for anthocyanin biosynthesis genes in the treated and untreated berries. For one group, consisting of chalcone synthase, flavanone-3-hydroxylase, dihydroxyflavonol-4-reductase and leucoanthocyanidin dioxygenase, the expression was inhibited or unchanged by the ethanol treatment, whereas for UDP glucose-flavonoid 3-O-glucosyltransferase (UFGT) there was a marked increase in expression from 1 to 20 days after ethanol treatment. These results suggest that the UFGT gene is a key factor in the observed anthocyanin accumulation following ethanol treatment.

Item Type:Article
HAL Id:hal-02064553
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Montpellier SupAgro (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > University of Adelaïde (AUSTRALIA)
Other partners > Université de Montpellier (FRANCE)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:12 Mar 2019 07:47

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