OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Impact of pH on the Stability and the Cross-Reactivity of Ochratoxin A and Citrinin

Bazin, Ingrid and Faucet-Marquis, Virginie and Monje, Marie-Carmen and El Khoury, Micheline and Marty, Jean-Louis and Pfohl-Leszkowicz, Annie Impact of pH on the Stability and the Cross-Reactivity of Ochratoxin A and Citrinin. (2013) Toxins, 5 (12). 2324-2340. ISSN 2072-6651

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
537kB

Official URL: https://doi.org/10.3390/toxins5122324

Abstract

Mycotoxins are secondary metabolites produced by several fungi contaminating crops. In several countries, the maximum permitted levels of mycotoxins are found in foodstuffs and feedstuffs. The common strategy of mycotoxin analysis involves extraction, clean-up and quantification by chromatography. In this paper, we analyzed the reasons of underestimation of ochratoxin A (OTA) content in wine, and overestimation of OTA in wheat, depending on the pH of the clean-up step and the simultaneous presence of citrinin (CIT). We demonstrated that the increase of pH by adding polyethylene glycol (PEG) to wine led to an underestimation of OTA by conversion of OTA into open ring ochratoxin A OP-OA. In comparing three methods of extraction and clean-up for the determination of OTA and CIT in wheat—(i) an inter-laboratory validated method for OTA in cereals using immunoaffinity column clean-up (IAC) and extraction by acetonitrile/water; (ii) a validated method using IAC and extraction with 1% bicarbonate Na; and (iii) an in-house validated method based on acid liquid/liquid extraction—we observed an overestimation of OTA after immunoaffinity clean-up when CIT is also present in the sample, whereas an underestimation was observed when OTA was alone. Under neutral and alkaline conditions, CIT was partially recognized by OTA antibodies

Item Type:Article
HAL Id:hal-02074103
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > ANABIOTOX (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > IMT Mines Alès (FRANCE)
Other partners > Université de Perpignan Via Domitia - UPVD (FRANCE)
Laboratory name:
Statistics:download
Deposited By: Loetitia MOYA
Deposited On:20 Mar 2019 13:35

Repository Staff Only: item control page