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Identification of multiple-derived peptides produced by Saccharomyces cerevisiae involved in malolactic fermentation inhibition

Rizk, Ziad and Rayess, Youssef El and Ghanem, Chantal and Mathieu, Florence and Taillandier, Patricia and Nehme, Nancy Identification of multiple-derived peptides produced by Saccharomyces cerevisiae involved in malolactic fermentation inhibition. (2018) FEMS Yeast Research, 18 (7). ISSN 1567-1364

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Official URL: https://doi.org/10.1093/femsyr/foy080

Abstract

An oenological strain of Saccharomyces cerevisiae was previously shown to produce a 5–10 kDa peptidic fraction responsible for the inhibition of malolactic fermentation (MLF). In the present study, we aim to further purify the anti-MLF peptides of this fraction. The yeast fermented synthetic grape juice medium was fractionated by ammonium sulfate precipitation combined with ultrafiltration. The 5–10 kDa fraction recovered at a saturation degree of 60%–80% was the only fraction that inhibited both the bacterial growth and the malate consumption in vivo. It also inhibited the malolactic enzyme activity in vitro at a pH range between 3.5 and 6.7. Therefore, it was purified by both anion and cation exchange chromatography. The eluates that inhibited the malolactic enzyme activity in vitro were migrated on Tricine SDS-PAGE and the protein bands were excised and sequenced by LC-MS/MS. The sequencing revealed nine peptides originating from eight proteins of S. cerevisiae. Two GAPDH cationic fragments of 0.9 and 1.373 kDa having a pI of 10.5 and 11 respectively, Wtm2p and Utr2p anionic fragments of 2.42 kDa with a pI of 3.5 and 4 respectively were thought to contribute the most to the MLF inhibition

Item Type:Article
HAL Id:hal-02057032
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Other partners > Lebanese University - LU (LEBANON)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Other partners > Holy Spirit University of Kaslik - USEK (LEBANON)
Other partners > Lebanese Agricultural Research Institute - LARI (LEBANON)
Laboratory name:
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Deposited By: Loetitia MOYA
Deposited On:05 Mar 2019 08:05

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