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Systematic error and comparison of four methods for assessing the viability ofSaccharomyces cerevisiae suspensions

Lange, Hélène and Bavouzet, Jean-Michel and Taillandier, Patricia and Delorme, C. Systematic error and comparison of four methods for assessing the viability ofSaccharomyces cerevisiae suspensions. (1993) Biotechnology Techniques, 7 (3). 223-228. ISSN 0951-208X

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Official URL: https://doi.org/10.1007/BF02566152

Abstract

Four methods for the determination of cell viability were compared: the plate count technique, the flow cytometer, and two microscopic numerations- one after methylene blue staining and the other one with epifluorescence. The experimental error of these techniques was for the first time estimated: 8% for both numerations under microscope and 13% for the plate count technique. The staining mechanisms were explained by comparing the numerations under microscope and the flow cytometer analysis.

Item Type:Article
HAL Id:hal-02143221
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: Michel Aressy
Deposited On:26 Feb 2019 12:42

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