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Production of xylanases by Bacillus polymyxa using lignocellulosic wastes

Pham, Phuong Lan and Taillandier, Patricia and Delmas, Michel and Strehaiano, Pierre Production of xylanases by Bacillus polymyxa using lignocellulosic wastes. (1998) Industrial Crops and Products, 7 (2-3). 195-203. ISSN 0926-6690

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Official URL: https://doi.org/10.1016/S0926-6690(97)00048-4

Abstract

Production of xylanolytic enzymes with no detectable cellulase activity was investigated using two strains of Bacillus polymyxa. The optimum pH and temperature for activities of the two xylanases ranged from 6.0 to 7.0 and from 45 to 50°C, respectively. The highest titres of xylanase, up to 24 nKat ml^-1 were produced within 36 and 42 h, respectively in shake flask cultures at 30°C. Enzyme production showed a cell growth associated profile. One of two strains, B. polymyxa CECT 153 was chosen for further detailed study. Numerous carbohydrates were examined for their ability to induce xylanase. It was found that xylan or xylan containing substrates, such as wheat straw, induced maximum and comparable levels of xylanase, while pure cellulose (avicel, a-cellulose) and the easily metabolisable sugars (glucose, sucrose) did not improve xylanase synthesis. Low levels of constitutive enzyme were produced as evidence from the culture medium without carbon source addition. Among various nitrogen sources tested, yeast extract was optimal for the production of xylanase.

Item Type:Article
HAL Id:hal-02143241
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: Michel Aressy
Deposited On:25 Feb 2019 14:22

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