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Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs

Pham, Phuong Lan and Taillandier, Patricia and Delmas, Michel and Strehaiano, Pierre Optimization of a culture medium for xylanase production by Bacillus sp. using statistical experimental designs. (1997) World Journal of Microbiology and Biotechnology, 14 (2). 185-190. ISSN 0959-3993

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Official URL: https://doi.org/10.1023/A:1008821827445

Abstract

The concentrations of oat spelt xylan, casein hydrolysate and NH4Cl in the culture medium for production of xylanase from Bacillus sp. I-1018 were optimized by means of response surface methods. The path of steepest ascent was used to approach the optimal region of the medium composition. The optimum composition of the nutrient medium was then easily determined by using a central composite design and was found to be 3.16g/l of xylan, 1.94g/l casein hydrolysate, 0.8g/l of NH4Cl. The xylanase production was increased by 135% when the strain was grown in the optimized medium compared to initial medium.

Item Type:Article
HAL Id:hal-02143245
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
Laboratory name:
Statistics:download
Deposited By: Michel Aressy
Deposited On:25 Feb 2019 13:33

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