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Physico-chemical characterization of the interaction of red fluorescent protein — DsRed with silica layers

Soumbo, Marvine and Pugliara, Alessandro and Monje, Marie-Carmen and Roques, Christine and Despax, Bernard and Bonafos, Caroline and Carles, Robert and Mlayah, Adnen and Makasheva, Kremena Physico-chemical characterization of the interaction of red fluorescent protein — DsRed with silica layers. (2015) In: 2015 IEEE Nanotechnology Materials and Devices Conference (NMDC), 13 September 2015 - 16 September 2015 (Anchorage, United States).

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Official URL: https://doi.org/10.1109/NMDC.2015.7439280

Abstract

The Discosoma Recombinant Red Fluorescent (DsRed) protein is the latest member of the family of fluorescent proteins. It holds great promise for applications in biotechnology and cell biology. However, before being used for rational engineering, knowledge on the underlying mechanisms relating the DsRed structural stability and adsorption properties on solid surfaces is highly demanded. The physico-chemical analysis performed in this study reveals that the interaction of DsRed with SiO2 surfaces does not lead to protein denaturation. The secondary structure of DsRed is preserved after adsorption and dehydration. The measured contact angles of sessile droplets with different DsRed concentrations determine the interaction as hydrophilic one. The photoluminescence emission of dehydrated DsRed droplets is found to be slightly red-shifted, peaking at 590 nm.

Item Type:Conference or Workshop Item (Paper)
HAL Id:hal-01763539
Audience (conference):International conference proceedings
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UPS (FRANCE)
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Deposited By: Loetitia MOYA
Deposited On:12 Nov 2018 12:39

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